全文获取类型
收费全文 | 27891篇 |
免费 | 2800篇 |
国内免费 | 5篇 |
出版年
2021年 | 431篇 |
2020年 | 222篇 |
2019年 | 338篇 |
2018年 | 319篇 |
2017年 | 327篇 |
2016年 | 599篇 |
2015年 | 968篇 |
2014年 | 1088篇 |
2013年 | 1350篇 |
2012年 | 1810篇 |
2011年 | 1825篇 |
2010年 | 1188篇 |
2009年 | 1022篇 |
2008年 | 1526篇 |
2007年 | 1553篇 |
2006年 | 1376篇 |
2005年 | 1363篇 |
2004年 | 1314篇 |
2003年 | 1330篇 |
2002年 | 1217篇 |
2001年 | 501篇 |
2000年 | 482篇 |
1999年 | 474篇 |
1998年 | 368篇 |
1997年 | 325篇 |
1996年 | 268篇 |
1995年 | 241篇 |
1994年 | 253篇 |
1993年 | 262篇 |
1992年 | 382篇 |
1991年 | 343篇 |
1990年 | 337篇 |
1989年 | 307篇 |
1988年 | 296篇 |
1987年 | 287篇 |
1986年 | 240篇 |
1985年 | 311篇 |
1984年 | 276篇 |
1983年 | 232篇 |
1982年 | 237篇 |
1981年 | 240篇 |
1980年 | 206篇 |
1979年 | 225篇 |
1978年 | 191篇 |
1977年 | 187篇 |
1976年 | 165篇 |
1975年 | 140篇 |
1974年 | 161篇 |
1973年 | 162篇 |
1972年 | 132篇 |
排序方式: 共有10000条查询结果,搜索用时 171 毫秒
1.
2.
In vivo EMG biofeedback was found to be an effective pedagogical tool for removing unwanted left-hand tension in nine violin and viola players. Improvement occurred rapidly and persisted throughout a 5-month follow-up period. Further studies will be necessary to assess the effect of biofeedback independent of placebo effects. The brevity of the method and the magnitude of improvement warrant further investigation. 相似文献
3.
4.
Transcriptional control, translation and function of the products of the five open reading frames of the Escherichia coli nir operon 总被引:3,自引:0,他引:3
Nerina R. Harborne Lesley Griffiths Stephen J. W. Busby Jeffrey A. Cole 《Molecular microbiology》1992,6(19):2805-2813
Five open reading frames designated nirB, nirD, nirE, nirC and cysG have been identified from the DNA sequence of the Escherichia coli nir operon. Complementation experiments established that the NirB, NirD and CysG polypeptides are essential and sufficient for NADH-dependent nitrite reductase activity (EC 1.6.6.4). A series of plasmids has been constructed in which each of the open reading frames has been fused in-phase with the beta-galactosidase gene, lacZ. Rates of beta-galactosidase synthesis during growth in different media revealed that nirB, -D, -E and -C are transcribed from the FNR-dependent promoter, p-nirB, located just upstream of the nirB gene: expression is co-ordinately repressed by oxygen and induced during anaerobic growth. Although the nirB, -D and -C open reading frames are translated into protein, no translation of nirE mRNA was detected. The cysG gene product is expressed from both p-nirB and a second, FNR-independent promoter, p-cysG, located within the nirC gene. No NADH-dependent nitrite reductase activity was detected in extracts from bacteria lacking either NirB or NirD, but a mixture of the two was as active as an extract from wild-type bacteria. Reconstitution of enzyme activity in vitro required stoichiometric quantities of NirB and NirD and was rapid and independent of the temperature during mixing. NirD remained associated with NirB during the initial stages of purification of the active enzyme, suggesting that NirD is a second structural subunit of the enzyme. 相似文献
5.
6.
7.
Field observations of two sympatric pitheciine species reveal that the positional repertoire of the white-faced saki, Pithecia pithecia, is dominated by leaping behaviors, whereas the bearded saki, Chiropotes satanas, is predominantly quadrupedal. Examination and comparison of the postcranial skeletal morphologies and limb proportions of these species display numerous features associated with their respective locomotor behaviors. These observations accord with associations found in other primate and mammalian groups and with predictions based on theoretical and experimental biomechanics. Preliminary observations of the skeletal morphology of Cacajao calvus demonstrate a marked similarity to that of Chiropotes. The fossil platyrrhine Cebupithecia sarmientoi displays greater similarity to Pithecia, suggesting that its positional repertoire also included significant leaping and clinging behaviors. 相似文献
8.
Escherichia coli RecBC pseudorevertants lacking chi recombinational hotspot activity 总被引:24,自引:7,他引:17 下载免费PDF全文
Pseudorevertants of an Escherichia coli exonuclease V (RecBC enzyme)-negative mutant have been isolated after ethyl methane sulfonate mutagenesis of a recC73 (presumed missense) mutant. The remedial mutations in each of the four pseudorevertants studied in detail map and complement as recC mutations. By several criteria, such as recombination proficiency, support of phage growth, RecBC nuclease activity, and cell viability, the pseudorevertants appear to have regained partially or completely various aspects of RecBC activity. However, chi recombinational hotspots, which stimulate exclusively the RecBC pathway of recombination, have no detectable activity in lambda vegetative crosses in the pseudorevertants. The properties of these mutants, in which the RecBC pathway of recombination is active yet in which chi is not active, are consistent with the hypothesis that wild-type RecBC enzyme directly interacts with chi sites; alternatively, the mutants may block or bypass the productive interaction of another recombinational enzyme with chi. 相似文献
9.
The ultrastructure of the calcareous skeleton is described in twenty–one species of recent tubuliporine cyclostome bryozoans, using field emission SEM. The succession of skeletal fabrics in interior walls may be classified into four different fabric suites. The first–formed part of the calcitic skeleton in all species for which it has been observed is a precursory fabric of tiny, wedge–shaped crystallites. This is succeeded in about half of the species studied by a fabric of transverse fibres, followed by foliated fabric and often semi–nacre (fabric suite 1). Most of the remaining species lack transverse fibres and have interior walls largely comprising semi–nacre (fabric suite 2). A few species have skeletons consisting of predominantly distally–oriented, irregularly or regularly foliated fabric (fabric suite 3). A single species has a skeleton of proximally–oriented foliated fabric (fabric suite 4). Basal exterior walls in all species have a precursory fabric of tiny wedge–shaped crystallites without a strong preferred orientation, deposited directly upon the organic cuticle, followed by a layer of planar spherulitic structure, which in turn is succeeded by a similar fabric to that developed in the interior wall of the species concerned. Outermost layers of frontal exterior walls exhibit one of the following combinations of three fabrics: an outer layer of (1) finely granular or wedge–shaped crystallites; a thin dense granular layer followed by (2) distally accreting planar spherulitic fabric., or (3) obliquely accreting planar spherulitic fabric growing partly towards the midline of the frontal wall. Terminal diaphragms usually have outer layers dominated by planar spherulitic ultrastructure with centripetal growth directions. The fabric suites present in tubuliporines encompass most known fabrics found in the other cyclostome suborders and support the notion that this species–rich suborder occupies a central position in cyclostome evolution. 相似文献
10.